lincRNA00907 promotes NASH progression by targeting miRNA-942-5p/TAOK1

• DOI: 10.18632/aging.205730
• Publication Date: 2024-04-13T02:44:31Z
• Authors: Gang Du; Zhaochen Jiang; Tong Xia; Mingkun Liu; Zeyang Liu; Huaxin Zhou; Hao Zhang; Xiangyu Zhai; Bin Jin
• Keywords: Steatohepatitis; Competing Endogenous RNA

Objective: The study aims to examine the involvement of lincRNA00907 in advancement non-alcoholic steatohepatitis (NASH). Methods: examination was conducted assess expression linc00907 liver tissues from NASH patients and healthy individuals. High-fat diets induced mouse models, while palmitic acid/oleic acid treatment used create vitro cell models. Various techniques, such as qRT-PCR, Oil Red O staining gene knockdown/overexpression, were impact on genes related lipid metabolism immunity, well intracellular accumulation. Furthermore, dual-luciferase reporter assays carried out confirm connection between miRNA-942-5p or TAOK1 mRNA. Results: Linc00907 found be significantly upregulated both Overexpression led an increase accumulation, knockdown resulted decreased content. It that binds with linc00907, their interaction confirmed assays. Additionally, predicted a downstream target miRNA-942-5p, upregulation due reversed by overexpression. overexpression reduces apoptosis but can knockdown. reduction counteracted associated accumulation lipids. Conclusions: Our research suggests functions competitive endogenous RNA (ceRNA) sequestering thus increasing encouraging hepatocytes, leading aggravation development. Targeting linc00907/miRNA-942-5p/TAOK1 axis may hold therapeutic potential for NASH.