MiR-328 targeting PIM-1 inhibits proliferation and migration of pulmonary arterial smooth muscle cells in PDGFBB signaling pathway

• DOI: 10.18632/oncotarget.10714
• Publication Date: 2016-07-19T21:34:53Z
• Authors: Zhengjiang Qian; Limin Zhang; Jidong Chen; Yanjiao Li; Kang Kang; Junle Qu; Zhiwei Wang; Yujia Zhai; Li Li; Deming Gou
• Keywords: Christian ministry

// Zhengjiang Qian 1, 2 , Limin Zhang 1 Jidong Chen Yanjiao Li Kang 3 Junle Qu Zhiwei Wang Yujia Zhai Deming Gou Shenzhen Key Laboratory of Microbial Genetic Engineering, College Life Sciences and Oceanography, University, Shenzhen, Guangdong, 518060, China Optoelectronic Devices Systems Ministry Education, Department Biochemistry Molecular Biology, School Basic Medical Sciences, 518000, Correspondence to: Gou, email: [e-mail] Keywords: miR-328, PASMCs, PDGF pathway, PIM-1 Received: April 13, 2016 Accepted: June Published: July 19, ABSTRACT MicroRNAs (miRNAs) have been recognized to mediate PDGF-induced cell dysregulation, but their exact functions remain be elucidated. By using a sensitive S-Poly(T) Plus qRT-PCR method, the expression profiling 1,078 miRNAs were investigated in pulmonary artery smooth muscle cells (PASMCs) with or without PDGFBB stimulation. MiR-328 was found as prominent down-regulated miRNA, displaying specific dose- time-dependent downregulation upon exposure. Functional analyses revealed that miR-328 could inhibit PASMCs proliferation migration both treatment. The Ser/Thr-protein kinase-1 (PIM-1) identified direct target functionally confirmed by rescue experiment. In addition, decrease might due increased DNA methylation transferase (DNMT1) methylation. Finally, serum level downregulated PAH patients associated congenital heart disease (CHD- PAH). Overall, this study provides critical insight into fundamental regulatory mechanism PDGFBB-activited via targeting PIM- implies potential circulating biomarker for CHD- diagnosis.