DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients

• DOI: 10.18632/oncotarget.13635
• Publication Date: 2016-11-26T07:53:48Z
• Authors: Noemí García-Romero; Josefa Carrión-Na...; Susana Esteban-Rubio; Elisa Lázaro-Ibáñez; María Peris-Celda; Marta M. Alonso; Juan Guzmán-De-Vi...; Carlos Fernández-...; Ana Ortiz de Mend...; Sara García-Duque; Carmen Escobedo-L...; Ricardo Prat-Acín; Cristóbal Belda-I...; Angel Ayuso-Sacido
• Keywords: Extracellular Vesicles; Extracellular vesicles; Exosome; Liquid biopsy

Tumor-cell-secreted extracellular vesicles (EVs) can cross the disrupted blood-brain barrier (BBB) into bloodstream. However, in certain gliomas, BBB remains intact, which might limit EVs release. To evaluate ability of tumor-derived to BBB, we used an orthotopic xenotransplant mouse model human glioma-cancer stem cells featuring intact BBB. We demonstrated that all types tumor cells-derived EVs-apoptotic bodies, shedding microvesicles and exosomes-cross be detected peripheral blood, provides a minimally invasive method for their detection compared liquid biopsies obtained from cerebrospinal fluid (CSF). Furthermore, these readily distinguished total murine EVs, since they carry human-specific DNA sequences relevant GBM biology. In small cohort glioma patients, finally blood cargo successfully detect presence IDH1G395A, essential biomarker current management glioma.