Macrophage production and activation are dependent on TRIM33

Lipopolysaccharides Chromatin Immunoprecipitation EMC OR-01 Pu.1 macrophage Trim33 Mice 03 medical and health sciences Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology Gene Regulatory Networks Myeloid Cells Cells, Cultured 0303 health sciences Macrophages DNA Sequence Analysis, DNA Macrophage Activation Chromatin 3. Good health RAW 264.7 Cells inflammation myeloid differentiation Research Paper Transcription Factors
DOI: 10.18632/oncotarget.13872 Publication Date: 2016-12-10T20:24:35Z
ABSTRACT
The tripartite motif (TRIM) family of proteins plays important roles in innate immunity and antimicrobial infection. None of these proteins has been shown to directly regulate transcription of genes in monocyte/macrophage except TRIM33 that we have recently shown to be a macrophage specific transcriptional inhibitor of Ifnb1. Using ChIP-seq analyses, we now report that TRIM33 is bound to two fold more genes in immature than in mature myeloid cell lines. When located near the same genes, TRIM33 is bound to different sequences in the two cell lines suggesting a role of TRIM33 in both immature and mature myeloid cells. Accordingly, expression of TRIM33 in immature myeloid cells is necessary for efficient production of small peritoneal macrophages, monocytes and bone marrow derived macrophage (BMDM) and TRIM33 targets a subset of genes involved in the inflammatory response only in mature myeloid cells. Functionally, this targeting is associated with impaired repression of pathways regulating the late phases of lipopolysaccharide (LPS) activation of BMDM and a high sensitivity to LPS in vivo when the trim33 gene is inactivated in mature myeloid cells. These findings pinpoint TRIM33 as an important transcriptional actor of monocyte/macrophage mediated inflammation.
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