Macrophage production and activation are dependent on TRIM33

• DOI: 10.18632/oncotarget.13872
• Publication Date: 2016-12-10T20:24:35Z
• Authors: Anne-Sophie Gallouet; Federica Ferri; Vanessa Petit; Aude Parcelier; Daniel Lewandowski; Nathalie Gault; Vilma Barroca; Stéphanie Le Gras; Eric Soler; Frank Grosveld; Irwin Davidson; Paul-Henri Romeo
• Keywords: Lipopolysaccharides; Chromatin Immunoprecipitation; EMC OR-01; Pu.1; macrophage; Trim33; Mice; 03 medical and health sciences; Animals; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; Gene Regulatory Networks; Myeloid Cells; Cells, Cultured; 0303 health sciences; Macrophages; DNA; Sequence Analysis, DNA; Macrophage Activation; Chromatin; 3. Good health; RAW 264.7 Cells; inflammation; myeloid differentiation; Research Paper; Transcription Factors

// Anne-Sophie Gallouet 1, 2, 3, 4, 5 , Federica Ferri 5, 6, * Vanessa Petit Aude Parcelier Daniel Lewandowski Nathalie Gault Vilma Barroca Stéphanie Le Gras 6 Eric Soler 7 Frank Grosveld Irwin Davidson Paul-Henri Romeo 1 CEA/DRF/iRCM/LRTS, 92265 Fontenay-aux-Roses cedex, France 2 Inserm U967, 3 Université Paris-Diderot, Paris 7, 4 Paris-Sud, 11, Equipe labellisée Ligue contre le Cancer, Department of Functional Genomics and Institut de Génétique et Biologie Moléculaire Cellulaire, CNRS/INSERM/ULP, BP 163, 67404 Illkirch Cedex, C. U. Strasbourg, Cell Biology, Erasmus Medical Center, DR Molenwaterplein 50, 3015GE, Rotterdam, The Netherlands These authors have contributed equally to this work Correspondence to: Romeo, email: [e-mail] Keywords: TRIM33, PU.1, macrophage, inflammation, myeloid differentiation Received: June 07, 2016 Accepted: November 12, Published: December 10, ABSTRACT tripartite motif (TRIM) family proteins plays important roles in innate immunity antimicrobial infection. None these has been shown directly regulate transcription genes monocyte/macrophage except TRIM33 that we recently be a macrophage specific transcriptional inhibitor Ifnb1 . Using ChIP-seq analyses, now report is bound two fold more immature than mature cell lines. When located near the same genes, different sequences lines suggesting role both cells. Accordingly, expression cells necessary for efficient production small peritoneal macrophages, monocytes bone marrow derived (BMDM) targets subset involved inflammatory response only Functionally, targeting associated with impaired repression pathways regulating late phases lipopolysaccharide (LPS) activation BMDM high sensitivity LPS vivo when trim33 gene inactivated findings pinpoint as an actor mediated inflammation.