Tissue Factor promotes breast cancer stem cell activity in vitro

• DOI: 10.18632/oncotarget.13928
• Publication Date: 2016-12-15T17:35:10Z
• Authors: Hudhaifah Shaker; Hannah Harrison; Robert Clarke; Goran Landberg; Nigel J. Bundred; Henri H. Versteeg; Cliona C. Kirwan
• Keywords: Anoikis

// Hudhaifah Shaker 1 , Hannah Harrison 2 Robert Clarke 3 Goran Landberg 4 Nigel J. Bundred Henri H. Versteeg 5 and Cliona C. Kirwan The University of Manchester, Manchester Academic Health Science Centre, Department Surgery, Hospital South UK Faculty Life Sciences, Breast Biology Group, Cancer Research Sahlgrenska Center, Gothenburg, Sweden Thrombosis Hemostasis, Einthoven Laboratory for Experimental Vascular Medicine, Leiden Medical Leiden, Netherlands Correspondence to: Kirwan, email: Keywords : cancer stem cells, tissue factor, breast Received May 23, 2016 Accepted August 25, Published December 13, Abstract cells (CSCs) are a subpopulation that can self-renew initiate tumours. clotting-initiating protein Tissue Factor (TF) promotes metastasis may be overexpressed in with increased CSC activity. We sought to determine whether TF activity vitro using human cell lines. expression was compared anoikis-resistant (CSC-enriched) unselected cells. In sorted into TF-expressing TF-negative (FACS), transfected knockdown (siRNA) overexpress (cDNA), by (i) mammosphere forming efficiency (MFE) (ii) holoclone colony formation (Hc) (iii) ALDH1 high ALDH1-activity T47D FACS T47Ds TF-overexpressing MCF7s had TF-low siRNA (MDAMB231,T47D) reduced control FVIIa MFE dose-dependent manner (MDAMB231, T47D). effects on were abrogated (T47D). CSCs ( ) demonstrate when selected expression, induced TF, stimulated (with FVIIa). Targeting the pathway vivo abrogate