CCL24 contributes to HCC malignancy via RhoB- VEGFA-VEGFR2 angiogenesis pathway and indicates poor prognosis

• DOI: 10.18632/oncotarget.14095
• Publication Date: 2016-12-27T19:25:59Z
• Authors: Lei Jin; Wei-Ren Liu; Meng-Xin Tian; Xi-Fei Jiang; Han Wang; Pei-Yun Zhou; Zhen-Bin Ding; Yuan-Fei Peng; Zhi Dai; Shuang-Jian Qiu; Jian Zhou; Jia Fan; Ying-Hong Shi
• Keywords: RHOB

CCL24 is one chemotactic factor extensively studied in airway inflammation and colorectal cancer but less hepatocellular carcinoma (HCC) retrospectively. So HCC tissue microarray (TMA) was used to estimate relationship between prognosis, cell experiments were conducted study its influence for biological behavior. injected nude mice monitor tumor formation pulmonary metastasis; qRT-PCR, western blot Immunohistochemistry explore potential mechanism. plays roles target cells via downstream CCR3, or it regulated by Type 2 helper T (Th2 cell) factors, so immune related conducted. Meanwhile, Rho GTPase family have close relation not only with priming, neovascularization; contributes neovascularization age-related macular degeneration family, Th2 Human Umbilical Vein Endothelial Cells uncover their trafficking. Ultimate validation confirmed small interfering RNA. Results showed expression higher caner tissues than adjacent normal tissues, could contribute proliferation, migration, invasion HCCs, accelerate metastasis, promote HUVECs tube formation. factors irrelevant HCCs; RhoB, VEGFA, VEGFR2 correlated both mRNA protein level. Downstream RhoB-VEGFA signaling pathway validated siRhoB siVEGFA inhibition. In a word, malignancy RhoB-VEGFA-VEGFR2 angiogenesis indicates poor which urges us further effects on diagnosis therapy HCC.