Elevated UHRF1 expression contributes to poor prognosis by promoting cell proliferation and metastasis in hepatocellular carcinoma

• DOI: 10.18632/oncotarget.14446
• Publication Date: 2017-01-03T12:40:46Z
• Authors: Xincheng Liu; Huohui Ou; Leyang Xiang; Xianghong Li; Yu Huang; Dinghua Yang
• Keywords: Male; 0301 basic medicine; Mice, Inbred BALB C; Carcinoma, Hepatocellular; Epithelial-Mesenchymal Transition; Liver Neoplasms; Mice, Nude; Apoptosis; Hep G2 Cells; Kaplan-Meier Estimate; Middle Aged; 3. Good health; G2 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Movement; Biomarkers, Tumor; CCAAT-Enhancer-Binding Proteins; Animals; Humans; Female; Research Paper; Aged; Cell Proliferation

// Xincheng Liu 1, 2 , Huohui Ou 1 Leyang Xiang Xianghong Li Yu Huang 3 Dinghua Yang Department of Hepatobiliary Surgery, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou, China The Second Shantou University College, Shantou, Laboratory Medicine, Correspondence to: Yang, email: [e-mail] Huang, [e-mail] Keywords: HCC, UHRF1, prognosis, proliferation, invasion Abbreviations: ubiquitin-like with plant homeodomain and ring finger domains, 1; hepatocellular carcinoma Received: May 17, 2016      Accepted: December 12, Published: January 02, 2017 ABSTRACT Ubiquitin-like (UHRF1) is overexpressed in a variety tumor tissues negatively correlated prognosis patients cancers, yet so far, comprehensive study UHRF1 (HCC) has not been conducted. present was designed explore the expression associated clinical implications, its possible functions HCC. Reverse transcription-polymerase chain reaction immunohistochemical staining were used detect HCC specimens including cancerous noncancerous tissues. Associations demographic clinicopathologic features analyzed, effects RNA interference on cell cycle, apoptosis, migration investigated vitro vivo . mRNA protein both upregulated patients. Furthermore, inhibition migration, invasion, epithelial-mesenchymal transition progression observed after knockdown, moreover, G2/M arrest detected cells. In conclusion, elevated contributes poor by promoting proliferation metastasis