A tree shrew glioblastoma model recapitulates features of human glioblastoma

• DOI: 10.18632/oncotarget.15225
• Publication Date: 2017-02-15T19:17:50Z
• Authors: Yaohui Tong; Junjun Hao; Qiu Tu; Hualin Yu; Lanzhen Yan; Yuan Li; Longbao Lv; Fei Wang; Antonio Iavarone; Xudong Zhao
• Keywords: Male; 0301 basic medicine; Gene Expression Profiling; Lentivirus; Tupaiidae; 3. Good health; Disease Models, Animal; 03 medical and health sciences; Animals; Humans; RNA Interference; Amino Acid Sequence; RNA, Small Interfering; Tumor Suppressor Protein p53; Glioblastoma; Transcriptome; Research Paper

Tupaia belangeri (tree shrew), an animal species whose genome has significantly higher similarity to primates than rodents, been used in biomedical research. To generate models that reproduce the human tumors more faithfully we present first report of a cancer model mimicking tumor genetics tree shrew. By engineering lentiviral system for transduction mutant H-Ras and shRNA against shrew p53, successfully generated malignant glioma The exhibited aggressive behavior relatively short latency, markedly reduced survival. Remarkably, biological features high-grade (necrosis, microvascular proliferation, pseudopalisading) were all glioma. Furthermore, genetic analysis revealed clustered within mesenchymal subgroup glioblastoma multiforme. Compared with corresponding mouse glioma, gliomas similar at gene expression profile. provides colleagues working field general accurate model.