Impact of VEGF, VEGFR, PDGFR, HIF and ERCC1 gene polymorphisms on thymic malignancies outcome after thymectomy

• DOI: 10.18632/oncotarget.4191
• Publication Date: 2015-09-15T06:28:55Z
• Authors: Rossana Berardi; Alessandro Brunelli; Silvia Pagliaretta; Vittorio Paolucci; Alessandro Conti; Gaia Goteri; Majed Refai; Cecilia Pompili; Giulia Marcantognini; Francesca Morgese; Zelmira Ballatore; Agnese Savini; Mariagrazia De Lisa; Miriam Caramanti; Matteo Santoni; Antonio Zizzi; Francesco Piva; Paola Mazzanti; Azzurra Onofri; Armando Sabbatini; Marina Scarpelli; Stefano Cascinu
• Keywords: ERCC1; Thymectomy

// Rossana Berardi 1 , Alessandro Brunelli 2 Silvia Pagliaretta Vittorio Paolucci Conti 4 Gaia Goteri 5 Majed Refai 3 Cecilia Pompili Giulia Marcantognini Francesca Morgese Zelmira Ballatore Agnese Savini Mariagrazia De Lisa Miriam Caramanti Matteo Santoni Antonio Zizzi Francesco Piva 6 Paola Mazzanti Azzurra Onofri Armando Sabbatini Marina Scarpelli Stefano Cascinu Medical Oncology, Università Politecnica delle Marche, Azienda Ospedaliero-Universitaria Ospedali Riuniti Umberto I – GM Lancisi G Salesi, Italy Department of Thoracic Surgery, St. James's University Hospital, Leeds, West Yorkshire, United Kingdom Urology, Ancona, Section Pathological Anatomy and Histopathology, Specialistic Clinical Odontostomatological Sciences, Correspondence to: Berardi, e-mail: [e-mail] Keywords: angiogenesis, prognosis, single nucleotide polymorphism, thymic epithelial tumor, tumor risk Received: April 04, 2015 Accepted: May 26, Published: June 08, ABSTRACT We aimed to analyze genotypes VEGF-A, VEGFR2, Flt4, PDGFRα, HIF-1α ERCC1 their correlation with patient outcome. DNA 57 consecutive patients (43 thymomas 14 carcinomas) who underwent total thymectomy at our Institution was extracted from paraffin-embedded tissue. selected polymorphisms in the following genes:HIF1-α (rs2057482T > C, rs1951795A rs2301113C A, rs10873142C T, rs11158358G rs12434438G rs11549465C rs11549467G A), VEGF-A (rs2010963G rs699947A C), VEGFR-2 (rs2305948C rs1870377T VEGFR-3 (rs307826T rs307821C PDGFR-α (rs35597368C T) (rs11615A G). Gene were determined by Real-Time PCR using TaqMan assays. As compared general population, allele frequency rs35597368T significantly higher (95% vs. 87%, p = 0.036), while alleles HIF1-α rs2057482C (78% 90%), rs1951795C (69% 87%), rs2301113A (70% 83%), rs10873142T rs11158358C (75% 88%), rs12434438A (67% 84%) lower. carcinomas (79% 72%, 0.0371). The factors correlated a longer overall survival: rs307826C, rs1870377A, rs35597368T/C, rs2301113C, rs2057482C/T, rs1951795C, rs11158358G/C rs10873142T/C, rs11615A ( < 0.05). Our results suggest, for first time, that PDGFR-α, SNPs are associated cancer survival.