Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device

• DOI: 10.18632/oncotarget.8232
• Publication Date: 2016-03-21T23:47:21Z
• Authors: Ting Yu; Zhe Guo; Hui Fan; Jing Song; Yuanbin Liu; Zhancheng Gao; Qi Wang
• Keywords: Cancer-Associated Fibroblasts

The tumor microenvironment is comprised of cancer cells and various stromal their respective cellular components. Cancer-associated fibroblasts (CAFs), a major part the cells, are key determinant in progression, while glucose-regulated protein (GRP)78 overexpressed many human cancers involved invasion metastasis. This study developed microfluidic-based three dimension (3D) co-culture device to mimic an vitro order investigate cell real-time. bionic chip provided significant information regarding role GRP78, which may be stimulated by CAFs, promote non-small lung vitro. data showed that CAF induced migration NSCLC A549 SPCA-1 this three-dimensional microdevice, confirmed using traditional Transwell system. Furthermore, GRP78 expression facilitate invasion, whereas knockdown blocked capacity. In conclusion, these indicated CAFs might up-regulation microdevice robust platform assess interaction environment study.