Microbial profile, antimicrobial resistance, and molecular characterization of diabetic foot infections in a university hospital

• DOI: 10.18683/germs.2021.1239
• Publication Date: 2021-04-19T16:30:36Z
• Authors: Azza A Ismail; Marwa A Meheissen; Talaat A Abd Elaaty; Nermine E Abd-All...; Heba S Kassab
• Keywords: Teicoplanin; Linezolid; Tazobactam

Diabetic foot infections (DFIs) are among the most severe complications of diabetes. The aim this study was to determine etiological pathogens DFIs in different Wagner's and IDSA/IWGDF grades, assess their antimicrobial susceptibility pattern together with molecular characterization antibiotic resistance genes.A prospective conducted on 120 DFI patients at Main Alexandria University Hospital, Egypt. aerobic anaerobic were determined using semi-quantitative culture PCR respectively. done according Clinical Laboratory Standards Institute guidelines. Detection carbapenemases class-1 integron genes carried out by polymerase chain reaction (PCR).A total 178 (124 aerobic, 54 anaerobic) identified from DFI, an average 1.82 isolates/subject. Among pathogens, Gram-negative predominated (98/124; 79%), which Pseudomonas spp. Proteus common. MRSA constituted more than 50% Gram-positive isolates. Polymicrobial infection found 42 (42.9%) subjects. proportion bacteria anaerobes increased grades severity. Multidrug extensively drug resistant isolates observed 86 (87.7%). 14 (11.7%) class 1 28 (23.3%) cases. Vancomycin, teicoplanin, linezolid effective agents against while colistin, imipenem, meropenem, piperacillin-tazobactam pathogens.Multidrug dominant all severity grades. However, decreased infection. clinical role our relatively high rate should be investigated. results could beneficial for designing future empiric protocols relation DFIs.