Evaluation of the effect of calcium silicate phosphate, osteon, bio_oss on cell viability and cell morphology of human osteoblast like cells (Saos2)

• DOI: 10.18869/acadpub.jrdms.1.3.8
• Publication Date: 2017-02-15T02:18:17Z
• Authors: S Sadatmansouri; ; N Ayubianmarkazi; ; S Aghajeri; ; MR Delaram; ; B Iranpour
• Keywords: Osteon; Morphology; Viability assay; Calcium silicate; Bioceramic

Background and Aim: Several reports have been published on the successful application of various bone substitute materials (BSM).Appropriate physiologic histologic characteristics reactions these against host cells are critically important.In this study, biocompatibility a new material has evaluated. Methods Materials:In experimental In vitro silicate calcium phosphate, Bio-Oss Osteon were compared by evaluation cell viability differentiation rate human osteoblast-like line (SaOS-2).No graft was used in control group.Cell evaluated MTT test after 1, 3 14 days incubation.Inverted Light Microscope SEM utilized for morphology.MTT morphology analyzed ANOVA all groups.Results: Cell group equaled 0.453±0.016,while groups it 0.453±0.016for Bio-Oss, 0.439±0.011for 0.425±0.026for phosphate.There no significant difference between groups.Spindle form dominant SaOS-2 groups.Conclusion: This study showed that phosphate appropriate comparable with Osteon.