Characterization of a cryptic plasmid from endophytic Enterobacter agglomerans isolated from Amazon’s Copaifera multijuga

• DOI: 10.19178/sci.amazon.v4i1.67-72
• Publication Date: 2015-12-14T18:56:39Z
• Authors: Alessandra Karisa...; Lucivana Prata So...; Enedina Nogueira ...; Carlos Gustavo Nu...; Edmar Vaz de Andrade; José Odair Pereira; Spartaco Astolfi-...
• Keywords: 0303 health sciences; 03 medical and health sciences

A small plasmid named pEA1 was isolated from Enterobacter agglomerans, an endophytic bacterium colonizing Copaifera multijuga (copaiba). The sequence determined by using the primer walking strategy and deposited in GenBank (accession number DQ659147). is a circular molecule with length of 2,545 bp. Sequence analysis showed this has GC content 34% (Guanine Citosine DNA sequence) contains four open reading frames (ORFs) capacity encoding more than 100 amino acids. ORF1, ORF2, ORF3 homology to mobilization proteins. ORF4 protein involved replication originating Pectobacterium atrosepticum.