[Components of drugs in acupoint sticking therapy and its mechanism of intervention on bronchial asthma based on UPLC-Q-TOF-MS combined with network pharmacology and experimental verification].

• DOI: 10.19540/j.cnki.cjcmm.20210903.401
• Publication Date: 2022-03-01
• Authors: Jun Hu; Ling Weng; Cong Zhang; Shu-Mei Zhao; Kaiwen Ge; Kuan DI; Meng Cao; Hesheng Wang; Lingang Zhao; Lanying Liu
• Keywords: KEGG; Calycosin; Fexofenadine

UPLC-Q-TOF-MS combined with network pharmacology and experimental verification was used to explore the mechanism of acupoint sticking therapy(AST) in intervention bronchial asthma(BA). The chemical components Sinapis Semen, Cory-dalis Rhizoma, Kansui Radix, Asari Radix et Zingiberis Rhizoma Recens were retrieved from TCMSP as self-built database. active AST drugs analyzed by UPLC-Q-TOF-MS, targets screened out Swiss-TargetPrediction. Targets BA collected GeneCards, intersection obtained Venny 2.1.0. potential imported into STRING DAVID for PPI, GO, KEGG analyses. asthma model induced house dust mite(HDM) established mice. on asthmatic mice explored pulmonary function, Western blot, flow cytometry. results indicated that 54 162 intersection. first 53 selected key targets. analyses showed presumedly acted SRC, PIK3 CA, other through such sinoacutine, sinapic acid, dihydrocapsaicin, 6-gingerol regulated PI3 K-AKT, ErbB, chemokine, sphingolipid, signaling pathways intervene pathological BA. can improve lung down-regulate expression K p-AKT proteins tissues, enhance PETN protein, reduce level type Ⅱ innate immune cells(ILC2 s) tissues In conclusion, may inhibit ILC2 s down-regulating K-AKT pathway relieve airway inflammation hyperresponsiveness.