[Anti-asthma components and mechanism of Kechuanting acupoint application therapy: based on serum metabolomics and network pharmacology].

• DOI: 10.19540/j.cnki.cjcmm.20220614.501
• Publication Date: 2022-12-01
• Authors: Shu-Mei Zhao; Xiao-Yan Gong; Jun Hu; Kuan DI; Jinjun Shan; Hesheng Wang; Cong Zhang; Lanying Liu
• Keywords: Orbitrap

This study aims to explore the anti-asthma components and mechanism of Kechuanting acupoint application therapy(KAAT) based on serum metabolomics network pharmacology. A total 60 asthma patients who had used low-dose inhaled corticosteroids-formoterol(ICS-formoterol) for a long time were randomized into western medicine group(low-dose ICS-formoterol) medicine+Kechuanting group(KAAT+low-dose ICS-Formoterol), 30 in either group. In addition, healthy people included as control(no intervention). The control test(ACT) score, forced expiratory volume 1 second(FEV1), peak flow(PEF) measured group before after treatment. potential biomarkers KAAT treatment screened by gas chromatography-mass spectrometry combined with multivariate analysis, related metabolic pathways further analyzed. UPLC/LTQ-Orbitrap-MS, together pharmacology, was employed construct component-target-pathway network. Thereby, effective me-chanism clarified. According ACT FEV1, PEF, asthma. 10 endogenous metabolomics, metabolism glycine, serine threonine, glyoxylic acid dicarboxylic obtained. UPLC/LTQ-Orbitrap-MS identified 51 chemical KAAT: 24 flavonoids, 11 alkaloids, 8 phenols, 2 diterpenoids, triterpenoids, glycosides, aldehydes. Network pharmacology analysis suggested that mainly acted crea-tinine(SRC), matrix metalloproteinase 9(MMP-9), other target proteins. closely pathway, phosphatidylinositol 3-kinase-protein kinase B(PI3 K-Akt), mitogen-activated protein kinase(MAPK), calcium signaling pathway. Sinapine thiocyanate, corydaline, dihydroberberine, stylopine, leonticine, N-methyl tetrahydroberberine, kaempferide, erio-dictyol, quercetin, catechin, 6-gingerol, 6-shogaol, ingenol, luteolin may be compounds preliminarily revealed It lays theoretical foundation in-depth clinical development application.