[Effect of Eucommiae Cortex extract mediated by ERβ/JNK pathway on learning and memory ability of APP/PS1 double-transgenic mice].

• DOI: 10.19540/j.cnki.cjcmm.20240903.401
• Publication Date: 2025-01-01
• Authors: Yue Li; Lili Zhang; Can Zhao; Hongmei Zhao; Yan Wang; Jiangjiang Fu; Jie Zhang; Ning Zhang; Hongdan Xu

To study the ameliorative effect of Eucommiae Cortex extract on spatial learning disabilities in APP/PS1 double-transgenic mice and explore its relationship with estrogen receptor β(ERβ)/c-Jun N-terminal kinase(JNK) signaling pathway, sixty 3-month-old male were randomly divided into a model group, an anti-brain failure capsule group(0.585 g·kg~(-1)), donepezil hydrochloride group(0.65 mg·kg~(-1)), group(1.3 15 C57BL/6 same genetic background set as WT control group. The memory ability was assessed by Morris water maze test(MWM), passive avoidance test(PAT), novel object recognition test(NOR). histomorphological cellular ultrastructural features hippocampal region observed hematoxylin-eosin(HE) staining transmission electron microscopy(TEM); molecular docking validation key active ingredients targets performed using AutoDock Vina software, immunohistochemical method(IHC) used to detect ERβ expression dentate gyrus(DG) area mouse hippocampus. Western blot(WB) utilized ERβ, p-JNK, JNK area. Compared those results behavioral experiments showed that latency group significantly increased, number platform traversals, target quadrant residence time decreased MWM. evasion reduced, errors increased PAT. index objects reduced NOR. HE indicated decrease neurons, disorganization pyramidal cell arrangement, nucleus consolidation, other changes. TEM some neuronal nuclei had consolidated state, slightly thickened aberrant nuclear membranes, fewer intracytoplasmic nidus bodies; IHC DG reduced. WB tissue decreased, p-JNK/JNK level elevated. significant latency, increase traversals MWM, PAT, In addition, there neurons mice. cells tended be arranged orderly manner; better state; elevated; elevated, effects terms behavioral, HE, indexes similar extract, no difference between experiments, estrogen-like components tightly bound ERβ. conclusion, binding receptors, regulation expression, activation ERβ/JNK pathway may one mechanisms which it improves