Design, Synthesis and Photophysical Studies of Improved Xanthene Dye to Detect Acetate

• DOI: 10.20944/preprints201810.0190.v1
• Publication Date: 2018-10-12T03:31:11Z
• Authors: Jose Manuel Paredes; Delia Miguel; Sandra Resa; Maria del Carmen ...; Yanira Diaz-Torres; Juan Manuel Cuerva; Luis Crovetto
• Keywords: physical_chemistry; 01 natural sciences; 0104 chemical sciences; 3. Good health

Development of biomarkers of analytes with interest in clinic is an important field of study. In this work, we synthesized and analyzed the new fluorescent acetate-biomarker, Iso-PG. The mechanism of detection is the acetate buffer mediated proton transfer reaction. The rate constants involved were obtained, and we measured the change in the fluorescence lifetime produced as a consequence of the presence of acetate in the medium. Finally, we checked its potential use as acetate biomarker in synthetic serum