Cell-Based Blood Biomarkers for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

• DOI: 10.20944/preprints202002.0029.v1
• Publication Date: 2020-02-04T04:05:26Z
• Authors: Daniel Missailidis; Oana Sanislav; Claire Allan; Sarah Annesley; Paul Fisher
• Keywords: Lymphoblast; Encephalomyelitis

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a devastating illness whose biomedical basis now beginning to be elucidated. We reported previously that, after recovery from frozen storage, lymphocytes (peripheral blood monocytic cells, PBMCs) ME/CFS patients die faster in culture medium than those healthy controls. also found that lymphoblastoid cell lines (lymphoblasts) derived these PBMCs exhibit multiple abnormalities mitochondrial respiratory function and signalling activity by the cellular stress-sensing kinase TORC1. These differences were correlated with disease severity, as measured Richardson Lidbury Weighted Standing Test. The clarity of between cells patient controls suggested they may provide useful biomarkers for ME/CFS. Here we report preliminary investigation into possibility using variety analytical classification tools, including linear discriminant analysis, logistic regression Receiver Operating Characteristic (ROC) curve analysis. results three different tests, lymphocyte death rate, TORC1 could each individually serve biomarker better 90% sensitivity but only modest specificity vís However, combination provided cell-based approaching 100% our sample. This level was almost equalled protocol which rate used highly sensitive test triage positive samples more time consuming expensive tests measuring lymphoblast activity. provides promising assist rapid accurate diagnosis