Sintered Glass Monoliths as New Supports for Affinity Columns

• DOI: 10.20944/preprints202103.0298.v1
• Publication Date: 2021-03-11T08:27:50Z
• Authors: Marco Wilke; Bettina Röder; Martin Paul; Michael G. Weller
• Keywords: Borosilicate glass; Porous glass

A novel stationary phase for affinity separations is presented. This material based on sintered borosilicate glass readily available as semi-finished filter plates with defined porosity and surface area. The shows fast binding kinetics excellent long-term stability under real application conditions due to lacking macropores high mechanical rigidity. can be easily modified standard organosilane chemistry immobilize selective binders or other molecules used biointeraction. In this paper, the manufacturing of columns their respective column holders by 3D printing shown in detail. model system protein A/IgG was chosen an example examine properties such monolithic realistic conditions. Several specifications, (dynamic) IgG capacity, pressure stability, performance, productivity, non-specific binding, peak shape, are It could that very separation speed, 250 mg antibody per hour collected, which surpasses productivity most same size. total capacity around 4 (5.5 mg/mL), sufficient tasks research laboratories. cycle time less than 1 minute. Due material's resistance, these compatible HPLC systems. usually not case columns, limited manual use low-pressure a also improves ability automation, enables purification hundreds cell supernatants one day. sharp shape elution leads enrichment effect, might increase concentration factor 3. final 7.5 mg/mL without need additional nanofiltration step. purity > 95% step nearly 99% second polishing run.