Assessment Of 3D Printed Polycaprolactone, Hydroxyapatite Nanoparticles and Polyethylene Glycol Diacrylate Scaffolds for Bone Regeneration

• DOI: 10.20944/preprints202210.0436.v1
• Publication Date: 2022-10-28T07:04:37Z
• Authors: Ana Catarina Sousa; Sara Biscaia; Rui Alvites; Mariana Branquinho; Bruna Lopes; Patrícia Sousa; Joana Valente; Margarida Franco; José Domingos Santos; Carla Mendonça; Luís Atayde; Nuno Alves; Ana Colette Maurício
• Keywords: Polycaprolactone

Notwithstanding the advances achieved in last decades field of synthetic bone substitutes, development biodegradable 3D scaffolds with ideal mechanical and biological properties remains an unattained challenge. In this work, a novel approach is explored to produce grafts mimicking complex structure using additive manufacturing. For first time, were produced, extrusion technique, composed thermoplastic polymer, polycaprolactone (PCL), hydroxyapatite nanoparticles (HANp), polyethylene glycol diacrylate (PEGDA). These further compared two groups scaffolds: one PCL another HANp. After production, optimisation characterisation these scaffolds, vitro evaluation was performed human dental pulp stem/stromal cells (hDPSCs). Through findings it possible conclude that PEGDA successfully produced presenting networks interconnected channels, hydrophilic (15.15 4.06°), adequate performance (10.41MPa 0.934), allowing cell viability significantly superior other analysed. To conclude, study demonstrated PCL, HANp may have promising effects on regeneration might open new insights for tissue substitutes.