Growth Developmental Defects of Mitochondrial Iron Transporter 1 and 2 Mutants in Arabidopsis in Iron Sufficient Conditions

• DOI: 10.20944/preprints202301.0097.v1
• Publication Date: 2023-01-05T08:04:15Z
• Authors: Joaquin Vargas; Isabel Gómez; Elena Vidal; Chun Pong Lee; A. Harvey Millar; Xavier Jordana; Hannetz Roschztta...
• Keywords: Pentatricopeptide repeat

Iron is the most abundant micronutrient in plant mitochondria and it has a crucial role biochemical reactions involving electron transfer. It been described Oryza sativa that Mitochondrial Transporter (MIT) an essential gene knockdown mutant rice plants have decreased amount of iron mitochondria, strongly suggesting OsMIT involved mitochondrial uptake. In Arabidopsis thaliana, two genes encode MIT homologues. this study, we analyzed different AtMIT1 AtMIT2 alleles, confirming individually nor are essential. When generated crosses between Atmit1 Atmit2 alleles were able to isolate homozygous double plants. Interestingly, obtained only when with T-DNA insertion intron region used for crossings, these cases correctly spliced mRNA was generated, although at low level. plants, which knockout AtMIT2, grown chacterised sufficient conditions. Pleiotropic developmental defects observed including abnormal seeds, increased number cotyledons, slow growth rate, pinoid stems, flower structures reduced seed set. We possible phenomenon suppression next generation correlating splicing containing T-DNA. Molecular analysis expression markers oxidative stress showed express degree perturbation. A RNA-Seq study performed could identify more than 760 differentially expressed Atmit2, transport, coumarin metabolism, hormones transport signaling. Our data suggest some phenotypes mediated by auxin homeostasis.