Biomineralization Process in Three-Dimensional Scaffolds Based on Bacterial Nanocellulose for Bone Tissue Engineering: Chemi-Cal and Morphological Features, and Stem Cell Differentiation

• DOI: 10.20944/preprints202302.0431.v1
• Publication Date: 2023-02-27T02:04:42Z
• Authors: Ana Cañas-Gutiérrez; Lenka Toro; Cristina Fornaguera; Salvador Borrós; Marlon Osorio; Cristina Castro-H...; David Arboleda-Toro
• Keywords: Bacterial Cellulose; Nanocellulose; Octacalcium phosphate; Von Kossa stain

Bacterial nanocellulose (BNC) surface has a negative charge that allows the adsorption of calcium ions to initiate nucleation different phosphate phases. The aim this study was investigate methods mineralization on three-dimensional microporous bacterial nanocellulose, mimetize composition, structure, and biomechanical properties natural bone. To generate 3D biomaterial, porogen particles were incorporated during BNC fermentation with strain Komagataeibacter medellinensis. Calcium phosphates (CPs) deposited scaffolds by five alternating immersing cycles solutions. Scanning electron microscopy micrograph showed have pore sizes, between 70 350 µm, but porous interconnectivity affected biomineralization method time. crystals are shown be rod-shaped ratio similar immature bone, increasing from 1.13 1.6 cycle numbers. main mineral phases obtained X-ray diffraction Octacalcium Dihydrogen Hexakis (phosphate (V)) Pentahydrate (OCP). In vitro cell studies good cellular adhesion higher viability 95% in all scaffolds. Osteogenic differentiation human bone marrow mesenchymal stem cells evaluated expression markers like alkaline phosphatase, osteocalcin, osteopontin. conclusion, it is possible prepare controlled microporosity, which could allow osteoblast adhesion, proliferation, differentiation.