Saliva Decreases Sucrose-induced Cariogenicity in an Experimental Biological Caries Model

• DOI: 10.20944/preprints202304.0824.v1
• Publication Date: 2023-04-25T00:52:45Z
• Authors: Rodrigo A Giacaman; Rodrigo Umaña; María José Nuñez; Natalia Díaz-Garrido; Constanza Echeverría; Natalia García-Ma...; Alex Mira; Constanza E Ferná...; Karla Gambetta-Te...; Carla P. Lozano
• Keywords: saliva; caries; biofilm; cariogenicity; <i>Streptococcus mutans</i>; Streptococcus mutans; saliva; cariogenicity; QH301-705.5; Biology (General); <i>Streptococcus mutans</i>; biofilm; Article; caries

Objective. Whether a minimum quantity of saliva is needed to inhibit the caries process unclear. Here, we evaluated effect dilutions on an in vitro model with Streptococcus mutans (S. mutans) biofilms. Methods. S. biofilms were grown enamel and root dentin slabs, culture media containing different proportions (v/v): 0%, 5%, 10%, 25%, 50%, 75% 100% saliva, exposed 10% sucrose solution (5 min, 3x/day), appropriate controls. After 5 (enamel) 4 (dentin) days, demineralization, biomass, viable bacteria polysaccharide formation analyzed. The acidogenicity spent was monitored overtime. Assays carried out triplicate 2 independent experiments (n=6). Results. An inverse relationship observed between acidogenicity, proportion, both dentin. A marked reduction demineralization when small amounts incorporated medium. For tissues, significant cells polysaccharides present, concentration-dependent manner. Conclusions. High quantities can almost completely sucrose-induced cariogenicity, but also exert dose-dependent caries-protective effect.