Methamphetamine Induces RIPK3 over Expression and Triggers of Akt-1/GSK3 Signaling Pathway in Amygdala in Postmortem User

• DOI: 10.20944/preprints202305.1949.v1
• Publication Date: 2023-05-29T02:18:12Z
• Authors: Zahra Azimzadeh; Samareh Omidvari; Somayeh Niknazar; Saeed Vafaei-Nezhad; Navid Ahmady Rooz...; Mohammad-Amin Abd...; Foozhan Tahmasebinia; Gholam-Reza Mahmo...; Hojjat Allah Abba...; Shahram Darabi
• Keywords: Meth-; Neurotoxicity; Methamphetamine

Methamphetamine (METH) has the potential to disrupt activities of neurotransmitters in Central Nervous System (CNS) and cause neurotoxicity through various pathways. These pathways include increased production reactive nitrogen oxygen species, hypothermia, induction mitochondrial apoptosis. In this study, we investigated long-term effects METH addiction on structural changes amygdala postmortem human brains, as well involvement CREB/BDNF Akt-1/GSK3 signaling We examined ten male comparing control subjects with chronic users, using immunohistochemistry, Real-time PCR (to measure levels CREB, BDNF, Akt-1, GSK3, TNF-α), Tunnel assay, stereology, assays for ROS, GSSG, GPX. The findings revealed that significantly reduced expression GPX, while increasing RIPK3, TNF-α. Furthermore, was found induce inflammation neurodegeneration amygdala, ROS mediated by