Rapid Prototyping of 3D Printed AgNPs and Nano-TiO₂ Embedded Hydrogels as Novel Devices with Multi-Responsive Antimicrobial Capability in Wound Healing

• DOI: 10.20944/preprints202305.1964.v1
• Publication Date: 2023-05-31T05:33:27Z
• Authors: Giulia Remaggi; Laura Bergamonti; Claudia Graiff; Maria Cristina Os...; Lisa Elviri
• Keywords: Biocompatibility; Viability assay; Silver nanoparticle; Titanium Dioxide

Two antimicrobial agents such as silver nanoparticles (AgNPs) and titanium dioxide (TiO2) have been formulated with natural polysaccharides (chitosan or alginate) to develop innovative inks for the rapid, customizable, extremely accurate manufacturing of 3D printed scaffolds useful dressings in treatment infected skin wounds. Suitable chemical-physical properties applicability these devices were demonstrated through evaluation water content (88-93%), mechanical strength (Young’s modulus 0.23-0.6 MPa), elasticity, morphology. 
 The tests performed against Staphylococcus Aureus Pseudomonas Aeruginosa activities Gram+ Gram– bacteria AgNPs TiO2 embedded CH ALG macroporous hydrogels (AgNPs MIC starting from 5 µg/ml). biocompatibility chitosan was widely by cell viability higher than that observed alginate. Constructs containing at 10 µg/ml concentration level did not significantly alter well presence dioxide; cytotoxicity towards human fibroblasts 100 µg/ml. In conclusions, here developed own features be cheap, highly defined, easy manufactured further applied personalized medicine applications.