Characterization of Nanoprecipitated PET Nanoplastics by ¹H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

• DOI: 10.20944/preprints202311.0087.v1
• Publication Date: 2023-11-02T07:56:20Z
• Authors: Milica Djapovic; Danijela Apostolovic; Vojislava Postic; Tamara Lujic; Vesna Jovanovic; Dragana Stanic-Vu...; Marianne Van Hage; Veselin Maslak; Tanja Cirkovic Ve...
• Keywords: Zeta potential; Sodium dodecyl sulfate

Manufactured nanoplastic particles (NPs) are indispensable for in vitro and vivo testing health risk assessment of this emerging environmental contaminant. High surface area inherent hydrophobicity plastic material, makes production NPs devoid any contaminants very challenging. In study we produced nanoprecipitated polyethylene terephthalate (PET) (300 nm hydrodynamic diameter) characterized presence ionic surfactant sodium dodecyl sulfate (SDS) by 1H NMR. Relative ratio NP/surfactant was monitored on the basis chemical shifts characteristic PET SDS. For a wide range surfactant/NP (1:17 to 1:1.2), measured zeta potential changed from -42.10 -34.93 mV, but no clear differences were observed cellular assays performed protein-rich media primary human cells. The remaining impurities contributed outcome biological applied protein-free buffers, such as red blood cells hemolysis. As size, shape, may all be relevant parameters effects NPs, relative quantification exemplified our work application NMR SDS could valuable auxiliary method quality control manufactured NPs.