Phytoene Desaturase (Pds) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)

• DOI: 10.20944/preprints202401.0588.v1
• Publication Date: 2024-01-09T00:34:47Z
• Authors: Fernanda dos Sant...; Marcelly Santana ...; Samantha Costa Bo...; Carla Catharina H...; Andresa Priscila ...; Anelita de Jesus ...; Julianna Matos da...; Leandro Eugenio C...; Tiago Antônio De ...; Claudia Fortes Fe...; Edson Perito Amorim
• Keywords: Phytoene synthase; Phytoene desaturase; Molecular marker

Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as selective marker for genome editing several species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable highly favorable. In Musa spp., the A increases fruit production quality, whereas B associated with tolerance to biotic abiotic stresses. objective of this study was identify molecular discriminate genomes banana. 2166 bp fragment ‘PDSMa’ identified polymorphic (identification accuracy 99.33%), ~332 ~225 fragments were detected ‘PDSMb’ primer 100% using MedCalc software. To best our knowledge, first use determine doses which will aid evaluating genomic constitution hybrids accessions at seedling stage, accelerating their classification crop genetic improvement programs.