The Distal Promoter of the <em>B438L </em>Gene<em> </em>of<em> </em>African Swine Fever Virus Is Responsible for the mRNA Transcription of the <em>B438L</em> and the Alternatively Spliced <em>B169L</em>

• DOI: 10.20944/preprints202405.0180.v1
• Publication Date: 2024-05-07T08:23:10Z
• Authors: Hongwei Cao; Hao Deng; Yanjin Wang; Diqiu Liu; Lian-Feng Li; Meilin Li; Dingkun Peng; Jingwen Dai; Jiaqi Li; Hua-Ji Qiu; Su Li
• Keywords: Transcription

The B169L protein (pB169L) of African swine fever virus (ASFV) is a structural with an unidentified function during the replication. sequences gene and downstream B438L are separated by short intergenic regions. However, regulatory mode transcription remains unknown. Here, we identified two distinct promoter regions start sites (TSS) located upstream open reading frame (ORF) B438L. Using reporter system, demonstrated that cis activity ORF proximal exhibited significantly higher levels compared distal in gene. Furthermore, transfection plasmids different promoters for could initiate expression HEK293T cells, also displayed activities promoter. Interestingly, initiated alternatively spliced mRNA (B169L mRNA2) encoding truncated pB169L (tpB169L) (amino acids 92–169), efficiency was increased upon mutation initiation codon Taken together, initiates both mRNA2. Comprehensive analysis transcriptional beneficial understanding association construction gene-deleted ASFV.