Epidemiology and Genetic Susceptibility of SARS-CoV-2 of Young Children Living in Northwestern Amazon Region Presenting Acute Gastroenteritis and Respiratory Infection

• DOI: 10.20944/preprints202407.2398.v1
• Publication Date: 2024-08-14T06:40:46Z
• Authors: Yan Cardoso Pimenta; Flávia Freitas de...; Carlos Eduardo da...; Bruno Loreto Aragão; Mauro França Silva; Alberto Ignácio O...; José Paulo Gaglia...; Isabella Fernande...; Marcia Terezinha ...
• Keywords: Molecular Epidemiology; Acute gastroenteritis

This study evaluated the epidemiological and genetic susceptibility profile caused by coronavirus 2 (SARS-CoV-2) in children ≤3 years of age with acute gastroenteritis (AGE) or respiratory infection (ARI), living northwestern region Amazon (NWAR). Fecal saliva sam-ples collected from 202 (n=404) were subjected to detection SARS-CoV-2 RT-qPCR. Polymorphisms angiotensin-converting enzyme (ACE rs4646994) ACE2 G8780A; rs2285666) genes detected SYBR GREEN real-time PCR PCR/Alul digestion, respec-tively. Secretory Lewis status histo-blood group antigen (HBGA) was performed using phenotyping/ELISA. Frequencies for AGE group: SARS-CoV-2; 8.9%; ARI 2.9%. The ACE I/I homozygous polymorphism gene majority. Males G8780A SNP more susceptible symptoms than (OR = 3.85; 95% CI-1.42-10.38; P 0.007). Most secretory FUT2 phenotype (92.6%). Surveillance diarrhea infections children, along understanding their causes, are crucial identifying preven-tion control strategies prioritizing developing new vaccines. Clinical manifesta-tions COVID-19 include AGE.