Populus trichocarpa EXPA6 Increases Salt Sensitivity by Facilitating Radial and Longitudinal Transport of Sodium Ions in Poplars

• DOI: 10.20944/preprints202408.0833.v1
• Publication Date: 2024-08-15T00:19:37Z
• Authors: Zhe Liu; Kexin Yin; Ying Zhang; Caixia Yan; Ziyan Zhao; Jing Li; Yi Liu; Bing Feng; Rui Zhao; Jian Liu; Kaiyue Dong; Jun Yao; Nan Zhao; Xiaoyang Zhou; Shaoliang Chen
• Keywords: Populus trichocarpa; Sodium salt; Salicaceae

Expansins are cell wall (CW) proteins that mediate the CW loosening and regulate salt tolerance in a positive or negative way. The transcription of Populus trichocarpa expansin A6 (PtEXPA6) was downregulated upon prolonged duration stress (48 h) after transient increase induced by NaCl (100 mM). role PtEXPA6 determined transferring gene into hybrid species alba × P. tremula var. glandulosa clone 84K (84K) INRA '717-1B4' (717-1B4). PtEXPA6-transgenic poplars 717-1B4 showed greater reduction photosynthesis, stem height diameter growth under reduced capacity for ROS scavenging antioxidant enzymes, which is associated with excessive Na+ accumulation roots shoots. NMT flux recordings radial translocation root xylem real-time from to leaves were than wild-type poplars. Analysis comparative contractility extensibility intact tips indicated increased transgenic 717-1B4. Noteworthy, PtEXPA6-promoted shown facilitate longitudinal transport We conclude overexpression leads facilitates subsequent leaves, resulting consequently