Interferon Signaling Activation and LINE1 Expression under Influence of Anticancer Plant Secondary Metabolites

• DOI: 10.20944/preprints202410.0853.v1
• Publication Date: 2024-10-12T00:31:50Z
• Authors: Olga Vlasova; Irina Antonova; Khamis Magomedova; Alena Osipova; Polina Shtompel; Anna Borunova; Tatyana Zabotina; Gennady Belitsky; Irina Budunova; Albert Jordan; Kirill Kirsanov; Marianna Yakubovs...
• Keywords: Expression (computer science)

Previously we discovered that among 15 DNA-binding plant secondary metabolites (PSMs) possessing anticancer activity, 11 compounds cause depletion of the linker histones H1.2 and/or H1.4 from chromatin fraction. Chromatin remodeling or multiH1 knocking-down is known to promote upregulation repetitive elements, ultimately triggering an interferon response. Herein, using HeLa cells and applying fluorescent reporter assay with flow cytometry quantitave RT-PCR, found PSMs depleting histones, 8 significantly activate type I signaling pathway. Out these resveratrol, berberine, genistein, delphinidin, naringenin curcumin also caused LINE1 expression detected by quantitate RT-PCR immunefluorescent staining analysis ORF1 γ-H2AX. Fisetin quercetin, which induced histone depletion, activated only signaling, but not expression. Curcumin, sanguinarine kaempferol, causing more intensively respectively H1.2, less without any changes For 4 PSMs, did observe effects interest. Thus, have shown for first time activation accompanies often impacts this activation.