Cytotoxic, Antioxidant, and Anti-genotoxic Properties of Combretastatin A4 in Human Peripheral Blood Mononuclear Cells: A Comprehensive In Vitro Study

• DOI: 10.20944/preprints202410.2520.v1
• Publication Date: 2024-11-01T01:15:18Z
• Authors: Petar Popović; Andrea Pirković; Dijana Topalović; Lada Živković; Milica Marković; Biljana Spremo-Po...
• Keywords: Scalable Vector Graphics

Despite significant progress in drug discovery and the promising antitumor potential of Combretastatin-A4 (CA-4) selectively targeting rapidly dividing cancer cells, its effects on non-dividing human such as peripheral blood mononuclear cells (PBMCs), remain unclear. This study aims to assess vitro bioactivity CA-4 PBMCs, with a focus antigenotoxic antioxidant properties, while comparing cytotoxic potency against cell lines (JAR HeLa), normal trophoblast line HTR-8/SVneo. Cell viability metabolic activity was assessed using MTT assay. ROS production PBMCs measured by H2DCFDA assay, DNA damage evaluated through comet exhibited cytotoxicity HTR-8/SVneo at concentrations above 200 µM, JAR HeLa, demonstrated 100 µM 1 respectively. also reduced levels under oxidative stress, showing across ranging from µM. Additionally, H2O2-induced up lower compared inhibited production, offering insights into therapeutic efficacy safety.