Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<em>Hermetia illucens</em>) Larvae; Comparison to Mealworm (<em>Tenebrio molitor</em>) Larvae

• DOI: 10.20944/preprints202410.2574.v1
• Publication Date: 2024-11-06T00:52:06Z
• Authors: Antoine Lecocq; Anna Luiza Farias...; Christina M. Lazov; Sheikh M. Rajiuddin; Anette Bøtner; Graham J. Belsham
• Keywords: Hermetia illucens

Insect larvae production offers the potential for large-scale synthesis of high quality protein that can be used as feed or food. However, currently, there are limitations on source substrates insect to use. One concern is survival animal pathogens within if their contaminated. In this study, a very stable virus, porcine parvovirus (PPV), mealworm (Tenebrio molitor) and black soldier fly (BSF) (Hermetia illucens) has been analyzed after oral ingestion virus. The PPV DNA genome could readily detected by PCR in both types up until 9 days post (DPI), end study period. Furthermore, infection susceptible PK15 cells from homogenized at least 3 dpi, using an immunoperoxidase staining method, DPI more sensitive real time assay. Thus, remain infectious during main growth phase through harvesting. it may considered exceptional respect since displays unusual stability, e.g. heat.