An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome
Trichome
Proteome
DOI:
10.20944/preprints202411.1045.v1
Publication Date:
2024-11-19T01:21:57Z
AUTHORS (9)
ABSTRACT
Cannabis sativa flower glandular trichomes (GTs) are the main site of cannabinoid synthesis. Phytohormones, such as jasmonic acid (JAS) and salicylic (SAL) have been shown to increase content in flowers, but how this is regulated remains unknown. This study aimed understand mechanisms action phytohormones on mature GT disc cells using an vitro assay. Live were isolated from a high-tetrahydrocannabinol cultivar incubated basal media plates supplemented with either kinetin (KIN), JAS, SAL, abscisic acid, ethephon, gibberellic brassinolide, or sodium diethyldithiocarbamate. Quantitative proteomic analysis revealed that KIN, JAS SAL caused greatest number changes cell proteome. Surprisingly, none treatments concertedly increased abundance related biosynthetic proteins GT, suggesting increases previous planta phytohormone studies likely due other processes, density. As well, treated GTs had numerous differentially abundant common. Several key for leucoplast differentiation, cuticular wax fatty metabolism primary regulation. Denoting cytokinin, signalling important coordinating differentiation development.
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