Integrative Analysis of Transcriptome and Metabolome Reveals the Light Quality-mediated Regulation of Adventitious Shoot Proliferation in Chinese Fir

• DOI: 10.20944/preprints202502.0888.v1
• Publication Date: 2025-02-14T05:07:59Z
• Authors: Meixiang Chen; Shanshan Xu; Yiquan Ye; Kaimin Lin; Weili Lan; Guangqiu Cao
• Keywords: Metabolome; Light intensity

Chinese fir (Cunninghamia lanceolata) is an important fast-growing tree species for timber production and ecological protection in China. Tissue culture a key method fast-propagating seedlings, but the technique hindered by low proliferation rate poor seedling quality. Light quality plays crucial role influencing growth, underlying regulatory mechanisms remain poorly understood. This study integrated transcriptome metabolome analyses to investigate how light qualities regulate adventitious shoot growth tissue-cultured seedlings. Seedlings were grown under red, green, blue, composite light-emitting diode (LED) conditions, with white serving as control. The results showed that blue blue-dominant significantly enhanced promoting higher levels of auxin (IAA) cytokinin (CTK), gibberellin (GA) strongly enhancing light. These conditions also chlorophyll content photosynthetic efficiency, thereby biomass accumulation, highlighting significant effects on growth. In contrast, red promoted height, leaf area, carotenoid content, likely due elevated GA reduced IAA CTK inhibited hormonal imbalances. Green increased abscisic acid (ABA) levels, counteracting proliferation-promoting effect zeatin riboside (ZR) suppressing Transcriptome identified pathways involved quality-mediated regulation, including plant hormone signal transduction, photosynthesis, flavonoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) four genes regulated further modulated biosynthesis signaling transduction. PSBS (encoding photosystem II protein) FTSH cell division protease) proliferation, accumulation. GID1 receptor) was essential height area expansion, GST glutathione S-transferase) played stress tolerance shoots. Differential expression GA- CTK-related genes, such GID1, DELLA (GA negative regulator), B-ARR A-ARR (Arabidopsis response regulators), underscored pivotal regulating pathways. research provided valuable insights optimizing would ultimately contribute sustainable plantation management.