Biodentine Stimulates Calcium-Dependent Osteogenic Differentiation of Mesenchymal Stromal Cells from Periapical Lesions

• DOI: 10.20944/preprints202503.0957.v1
• Publication Date: 2025-03-14T02:35:46Z
• Authors: Mile Eraković; Marina Bekić; Jelena Đokić; Sergej Tomić; Dragana Vučević; Luka Pavlović; Miloš Duka; Milan Marković; Dejan Bokonjić; Miodrag J Čolić

Biodentine, a tricalcium silicate cement, has emerged as retrograde root-end filling material to promote periapical lesion (PL) healing after apicoectomy. However, its underlying mechanisms remain unclear. This study tested the hypothesis that Biodentine stimulates osteogenic differentiation of mesenchymal stromal cells (MSCs) derived from PLs. The extract (B-Ex) was prepared following ISO 10993-12 by incubating polymerized in RPMI medium (0.2 g/mL) for three days at 37 °C. B-Ex, containing both released microparticles and soluble components, incubated with PL-MSCs cultured either basal MSC or suboptimal medium. Osteogenic assessed Alizarin Red staining expression 20 osteoblastogenesis-related genes, including transcription factors, signaling molecules, extracellular matrix proteins, bone hormones, cytokines/cytokine receptors, remodeling factors. Non-cytotoxic concentrations B-Ex stimulated proliferation induced their dose-dependent manner, significantly enhanced effect process prolonged relative optimal conditions. stimulatory primarily calcium-dependent, it reduced 85% when treated calcium-chelating agent EGTA. In conclusion, promotes calcium-dependent supporting role healing.