Neuroprotective and Anti-Inflammatory Activity of <i>Undaria pinnatifida</i> Fucoidan <i>In Vivo</i>—A Proteomic Investigation

• DOI: 10.20944/preprints202503.1193.v1
• Publication Date: 2025-03-20T01:14:06Z
• Authors: Cheng Yang; Corinna Dwan; Barbara Wimmer; Maurizio Ronci; Richard Wilson; Luke Johnson; Vanni Caruso
• Keywords: Fucoidan

Undaria pinnatifida fucoidan (UPF), a bioactive sulphated polysaccharide, is widely recognised for its anti-inflammatory, antioxidant, antitumor, anticoagulant, antiviral, and immunomodulatory properties. However, the precise mechanisms by which UPF regulates inflammation neuronal health remain unclear. This study aimed to investigate effects of supplementation on pro-inflammatory cytokines in skeletal muscle, small intestine, hypothalamus, as well plasma cytokine levels. Additionally, brain proteomic investigation nucleus accumbens (NAc) was performed assess UPF’s impact protein expression mice. A total 64 C57BL/6J mice were administered either standard chow or high fat diet (HFD) with without (400 mg/kg/day) 10 weeks. In HFD-fed mice, significantly reduced (TNF-α, IL-1β, IL-6) while also lowering circulating IL-1α IL-6 Proteomic analysis NAc revealed that modulated proteins involved oxidative stress, neuroinflammation, neurotransmitter regulation, endoplasmic reticulum stress. contrast, chow-fed had no effect neuroinflammatory-oxidative stress markers but influenced abundance associated immune response innate immunity. These findings highlight diet-dependent UPF, suggesting an adaptive role modulating stress-response pathways under different metabolic conditions highlighting potential neuroprotective support inflammation-related disorders health.