QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS

• DOI: 10.21010/ajtcam.v14i2.21
• Publication Date: 2017-03-29T10:59:47Z
• Authors: Prawez Alam; Ahmed I. Foudah; Hala H. Zaatout; Y T Kamal; Maged S. Abdel-Kader
• Keywords: Glycyrrhizin; Glycyrrhiza; Liquiritin; Liquiritigenin

Background: A simple and sensitive thin-layer chromatographic method has been established for quantification of
 glycyrrhizin in Glycyrrhiza glabra rhizome baby herbal formulations by validated Reverse Phase HPTLC method.
 Materials Methods: RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC
 plates methanol-water (7: 3 v/v) as mobile phase.
 Results: The developed plate scanned quantified densitometrically at 256 nm. Glycyrrhizin peaks from
 were identified comparing their single spot Rf = 0.63 ±
 0.01. Linear regression analysis revealed a good linear relationship between peak area amount of the
 range 2000-7000 ng/band.
 Conclusion: validated, accordance ICH guidelines precision, accuracy, robustness. The
 proposed will be useful to enumerate the therapeutic dose well bulk
 drug.