Androgen attenuates antitumor effects of gastric cancer cells by bone marrow mesenchymal stem cells via restricting the JNK signaling activation

• DOI: 10.21037/tcr.2019.06.04
• Publication Date: 2019-06-28T12:08:17Z
• Authors: Linsong Mu; Wu Sui; Yang Lin; Wentao Yu; Hailong Su; Xiang Yu; Chengkun Qin
• Keywords: Viability assay

Researches on bone marrow mesenchymal stem cells (BMMSCs) have generated controversial results in tumor research. In the present study, we aimed to explore functions of BMMSCs gastric cancer and possible mechanism a mimicking microenvironment stomach.Transwell co-cultured system was used co-culture SGC-7901 cells. some experiments, androgen its antagonist were added into as required. Cell viability, cell apoptosis, mRNA protein expressions apoptosis- JNK signaling- associated genes respectively determined by performing counting kit-8, flow cytometry, quantitative real-time PCR western blot.Androgen contributed growth system, not only suppressed induced apoptosis promoted necrosis factor (TNF)-α release, but also regulated level Bax/Bcl-2 elevated phosphorylation (p)-JNK p53. After adding androgens, anti-tumor effects weakened. Meanwhile, antagonists androgens could partially recover vitro inhibitory activation signaling.This study demonstrated important roles vitro. Additionally, stomach, weakened antitumor limiting signaling activation, suggesting that may be promising adjuvant drug therapy.