Tertiary lymphoid structure patterns predicted anti-PD1 therapeutic responses in gastric cancer

• DOI: 10.21147/j.issn.1000-9604.2022.04.05
• Publication Date: 2022-09-08T03:22:19Z
• Authors: Quan Jiang; ; Chenyu Tian; Hao Wu; Lingqiang Min; Hao Chen; Lingli Chen; Fenglin Liu; Yihong Sun
• Keywords: Microsatellite Instability; Cancer Immunotherapy

Recent studies have highlighted the distinct value of tertiary lymphoid structure (TLS) for immunotherapeutic response prediction. However, it remains unclear whether TLS could play such roles in gastric cancer (GC).In this study, tumor tissue slices from 292 GC patients Zhongshan Hospital were firstly reviewed to explore correlation between and clinical characteristics. Subsequently, we curated 38 reported genes that may function as triggers performed consensus molecular subtyping public RNA-seq datasets determine patterns GC. Based on differentially expressed acquired two patterns, quantified TLS-related principal component analysis (PCA) algorithm develop score. A immunotherapy cohort including 13 who received programmed cell death 1 (PD1) blockade therapy was established conduct RNA sequencing multiplex immunohistochemistry (mIHC) tests using formalin-fixed paraffin-embedded (FFPE) tissues. The corresponding score immune counts further compared based therapeutic variations.Mature revealed an independent prognostic factor patients. Patients with higher characterized by prolonged survival time superior immunotherapy. correlated immunotherapy-related characters, microsatellite instability (MSI) mutation burden (TMB). In addition, data indicated a PD1 therapy. mIHC also PD1+CD8+ T significantly increased high-TLS group.This study associated landscape diversity complexity. Quantitatively evaluating individual will strengthen our understanding TME characteristics promote more effective strategies.