Microscopic Measurement of Circular Dichroism Spectra

• DOI: 10.2116/analsci.24.297
• Publication Date: 2008-02-12T05:49:43Z
• Authors: Aira Matsugaki; Hideaki Takechi; Hideaki Monjushiro; Hitoshi Watarai
• Keywords: Phaseolus; Plant Leaves; Circular Dichroism; Membranes, Artificial; DNA; Equipment Design; Microscopy, Polarization; Particle Size; Sensitivity and Specificity; 01 natural sciences; 0104 chemical sciences

A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 x 60 microm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.