Immunohistochemistry for phospho-myosin light chain 2 in adult murine skin

• DOI: 10.21203/rs.2.10178/v1
• Publication Date: 2019-07-30T12:50:50Z
• Authors: Martim Dias Gomes; Soriba Letzian; Michael Saynisch; Sandra Iden
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract Phosphorylation of the myosin regulatory light chain 2 is a post-translational modification commonly used to report for myosin ATPase activity and actomyosin contractility. While its use in cell culture has been broadly reported in several studies, data on immunostaining in tissues has been sparse and inconsistent. In this protocol we report a methodology to stain phospho-myosin light chain 2 (pMLC2 Ser19) and double phospho-myosin light chain 2 (ppMLC2Thr18/ Ser19) in adult murine epidermis.