Identification of quantitative trait loci and the exploration of candidate genes for the tolerance to Zn deficiency in maize

• DOI: 10.21203/rs.2.12668/v1
• Publication Date: 2019-08-13T01:53:58Z
• Authors: Jianqin Xu; Xiaoyang Zhu; Xiuyi Fu; Futong Yu
• Keywords: Candidate gene; Genome-wide Association Study

Abstract Background Zn is essential for plants and deficiency leads to great reduction in quality quantity of crops. Maize, as one the most important main staple crops worldwide, more susceptible than any other cereal Therefore, understanding functional mechanisms tolerance maize urgent but still lacking. In this study, quantitative trait loci (QTL) analysis K22 By815 RIL population with high-density bin map was conducted investigate genetic basis tolerate deficiency, subsequently some candidate genes were identified considered being associated metabolisms plants. Results 21 QTLs detected accounted 5.9% - 16.6% phenotypic variations. Based on co-localization study comparisons previous studies different GWAS populations, 223 inside reduced QTL peak intervals chromosome 1, 2, 6, 7 9. Furthermore, 9 within bins valuable genomic regions are suggested be ions transportation redox processes affected by deficiency. Additionally, 5 genes, including ZmIRT1, ZmNRAMP6, ZmEIN2 ZmHMAs, whose homologous gene have been studied responsible metal cations ethylene-signaling pathway requiring a transition discovered we mapped. Conclusions 14 target mapped work explored elucidate potential functions homeostasis direct or indirect effects maize. It first time that ZmHMAs using linkage under maize, providing evidence foundation further characterization. Our findings assisted us untangling possible response