Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in Pteris cretica var. Albo-lineata hyperaccumulator

• DOI: 10.21203/rs.2.13257/v1
• Publication Date: 2019-08-21T01:01:58Z
• Authors: Veronika Zemanová; Marek Popov; Daniela Pavlíková; Pavel Kotrba; František Hnilička; Jana Česká; Milan Pavlík
• Keywords: Frond; Pteris vittata

Abstract Background Arsenic (As) toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus this paper was on the relationship between As-induced long-term stress and plant senescence hyperaccumulator Pteris cretica var. Albo-lineata ( Pc -Al).Results We showed that -Al grown pots haplic chernozem contaminated with 100 mg As kg -1 (As ) for 122 days could accumulate more than 2000 2800 dry matter old young fronds, respectively. Analysis 5-methylcytosine (5mC) confirmed overall methylation status fronds ferns reduced contrast to control treatment. Compared controls, (by 6% 10% fronds); however, decrease significant only fronds. correlations 5mC, direct toxicity, decreases chlorophylls, fluorescence photosynthetic rate be affected by epigenetic changes. Photosynthetic processes were determined treatment reduction gas-exchange parameters, carotenoids chlorophylls 5% 26%, respectively). Hyperaccumulation resulted elevation all analysed nutrients (Cu, Mn, Zn, Mg, S) but not fronds.Conclusions results point complex changes metabolism -Al, upon exposure contamination. most impact found physiological parameters correlated significantly toxicity. Our analysis very low water potential values lignification cell walls roots transports assimilated metabolites reduced.