Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

• DOI: 10.21203/rs.2.13257/v4
• Publication Date: 2020-02-21T19:11:09Z
• Authors: Veronika Zemanová; Marek Popov; Daniela Pavlíková; Pavel Kotrba; František Hnilička; Jana Česká; Milan Pavlík
• Keywords: Frond; Pteris vittata; Arsenic toxicity

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus this paper was on the relationship between As-induced stress and plant senescence hyperaccumulator Pteris cretica var. Albo-lineata (Pc -Al). We assume difference physiological parameters level methylation young old fronds as symptoms As toxicity. Results accumulation Pc -Al fronds, grown pots haplic chernozem contaminated with 100 mg kg -1 for 122 days, decreased age. Content higher than fronds. highest content determined 250 -1. increase age confirmed nutrients – Cu, Mg, Mn, S Zn. A significant elevation analysed showed affected status but 5-methylcytosine (5mC) only (from 25% to 12%). Determined photosynthetic processes decrease fluorescence, rate chlorophylls treatments both Water potential by Thinning sclerenchymatous inner cortex reduction average tracheid metaxylem vascular cylinder roots treatment. Irrespective age, positively correlated 5mC while negatively direct Opposite results were found contents Conclusions point changes metabolism -Al, upon low high exposure contamination. impact correlations Our analysis very water values lignification cell walls that transports assimilated metabolites reduced. our results, epigenetic could affect studied especially