Identification of Dissociation Factors in Pancreatic Cancer using a Mass Spectrometry-Based Proteomic Approach

• DOI: 10.21203/rs.2.13610/v2
• Publication Date: 2019-12-11T17:20:50Z
• Authors: Peng Liu; Lingming Kong; Keke Liang; Yunhao Wu; Haoyi Jin; Bing Song; Xiaodong Tan
• Keywords: Proteomics; Proteome; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Pancreatic cancer; Secreted proteome; SEC; 3. Good health; Gene Expression Regulation, Neoplastic; Pancreatic Neoplasms; Survival Rate; Tandem Mass Spectrometry; Cell Line, Tumor; Mass spectrum; Biomarkers, Tumor; Disease Progression; Humans; Databases, Protein; RC254-282; Research Article; Chromatography, Liquid

Abstract Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. In this study, different protein pretreatment methods were used to analyze the secretome, furthermore to identify dissociation factors in pancreatic cancer. Pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. YWHAG could be used as a prognostic indicator for pancreatic cancer. Finally, the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells, and the results might be used to identify biomarkers in liquid biopsy.