Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

• DOI: 10.21203/rs.2.14772/v3
• Publication Date: 2020-01-14T13:04:21Z
• Authors: Keiko Yamamoto; Shunsuke Ohnishi; Takeshi Mizushima; Junichi Kodaira; Masayoshi Ono; Yutaka Hatanaka; Kanako Hatanaka; Yugo Kuriki; Mako Kamiya; Nobuyuki Ehira; Keisuke Shinada; Hiroaki Takahashi; Yuichi Shimizu; Yasuteru Urano; Naoya Sakamoto
• Keywords: Dipeptidyl peptidase

<title>Abstract</title> Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated become after cleavage a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed reported useful for detection squamous cell carcinoma head neck, esophagus; however, there lack studies focuses on detecting EGJ by fluorescence molecular imaging. Therefore, we investigated visualization applying EP-HMRG clinical samples resected endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with was performed in 21 ESD, intensity tumor non-tumor regions interest prospectively measured. Immunohistochemistry also determine expression DPP-IV. Results: showed lesions were visualized within few minutes application EP-HMRG, sensitivity, specificity, accuracy 85.7%, respectively. However, tumors background intestinal metaplasia did not have sufficient contrast-to-background ratio since complete expresses measurements revealed all expressed Conclusions: could do metaplasia.