A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring

• DOI: 10.21203/rs.2.14955/v1
• Publication Date: 2019-09-25T00:46:24Z
• Authors: Lihua Wang; Shijiang Mi; Rachel Madera; Llilianne Ganges; Manuel V. Borca; Jingqiang Ren; Chase Cunningham; Ada G. Cino-Ozuna; Hongwei Li; Changchun Tu; Wenjie Gong; Jishu Shi
• Keywords: Strain (injury)

Abstract Background: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation CSF vaccines. However, VNT a time consuming procedure that requires cell culture live virus manipulation. C-strain vaccine the most frequently control prevention. In this study, we presented neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with emphasis on replacement post–vaccination monitoring. Results: One (6B211) which has potent activity against was generated. A novel ELISA established optimized strategy 6B211 can compete induced antibodies in pig serum to bind capture antigen E2. By testing negative sera (n=445) positive (n=70), cELSIA showed 100% sensitivity (95% confidence interval: 94.87 100%) specificity 100 100%). (n=139) parallel, cELISA excellent agreement (95.7%) VNT. The inhibition rate samples highly correlated their titers (r 2 =0.903, p<0.001). addition, intra- inter-assays exhibited acceptable repeatability low coefficient variations (CVs). Conclusions: This demonstrated high level correlation It reliable tool sero-monitoring vaccination campaign because it rapid, simple, safe cost effective be monitor vaccination-induced immune response at population level.