The Expression and Significance of mTORC1 in Diabetic Retinopathy

• DOI: 10.21203/rs.2.18087/v1
• Publication Date: 2019-12-04T21:42:01Z
• Authors: Yanli Liu; Yarong Zheng; Yekai Zhou; Yi Liu; Mengjuan Xie; Wenjing Meng; Meixia An
• Keywords: Intraperitoneal injection; PEDF

Abstract Background: To investigate the expression and significance of mechanistic target rapamycin complex 1(mTORC1) in diabetic retinopathy(DR), to find new targets methods for treatment DR.Methods: A DR rat model was prepared by general feeding combined with intraperitoneal injection 10% streptozotocin (60 mg/kg). The rats were randomly divided into a control group (NDM group) diabetes (DM group).Three months later,the degrees retinopathy determined using hematoxylin eosin staining,and levels p-S6, VEGF, PEDF proteins detected immunohistochemistry western blotting. Human retinal capillary endothelial cells (HRCECs) cultured high glucose conditions,then treated or transfected siTSC1.The protein p-S6 assessed 5-ethynyl-2´-deoxyuridine assay used detect cell proliferation, Transwell migration.Results: DM successfully developed. expressions VEGF significantly increased (p < 0.05), decreased compared 0.05). In vitro,the glucose(HG) induced HRCECs normal proliferation migration glucose(NG) After transfection siTSC1 activate mTORC1,the increased,as well as migration.In contrast HG HRCECs, decrased 0.05).Conclusion: mTORC1 played an important role DR. activation, protein, regulated proteins, changed cells.The can therefore be target,as