The Expression and Significance of mTORC1 in Diabetic Retinopathy

• DOI: 10.21203/rs.2.18087/v2
• Publication Date: 2020-02-25T18:34:06Z
• Authors: Yanli Liu; Yarong Zheng; Yekai Zhou; Yi Liu; Mengjuan Xie; Wenjing Meng; Meixia An
• Keywords: PEDF; Intraperitoneal injection

Abstract Background: To investigate the expression and significance of mechanistic target rapamycin complex 1(mTORC1) in diabetic retinopathy(DR), to find new targets methods for treatment DR. Methods: A DR rat model was prepared by general feeding combined with intraperitoneal injection 10% streptozotocin (60 mg/kg). The rats were randomly divided into a control group (NDM group) diabetes (DM group). Three months later, degrees retinopathy determined using hematoxylin eosin staining, levels p-S6, VEGF, PEDF proteins detected immunohistochemistry western blotting. Human retinal capillary endothelial cells (HRCECs) cultured high glucose(HG) conditions, then treated or transfected siTSC1.The protein p-S6 assessed 5-ethynyl-2´-deoxyuridine assay used detect cell proliferation, Transwell migration. Results: DM successfully developed. expressions VEGF significantly increased (p < 0.05), decreased compared NDM 0.05). In vitro, protein, as well proliferation migration, HG induced HRCECs 0.05) (normal glucose) After transfection siTSC1 activate mTORC1, increased. contrast, expression, Conclusion: mTORC1 plays an important role activation, regulated proteins, changed migration cells. can therefore be target,as